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Biodetection Technologies for First Responders


2015

Biodetection Technologies for First Responders: 2015 edition. PCR-based Detection Systems: GeneReach USA:  POCKIT™
. p 5.11-12. Pacific Northwest National Laboratory.(PNNL)


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2014

Biodetection Technologies for First Responders: 2014 edition. PCR-based Detection Systems: GeneReach USA: POCKIT™. p 5.10. Pacific Northwest National Laboratory.(PNNL)


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SCI Publications


2018

Vissani M.A., Tordoya M.S., Tsai Y.-L., Lee P.-Y.A., Shen Y.-H., Lee F.-C., Wang H.-T.T., Parreño V., Barrandeguy M. 2018. On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions. Journal of Virological Methods 257 (2018) 29–32 


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Jih-Jin Tsai, Li-Teh Liu, Ping-Chang Lin, Ching-Yi Tsai, Pin-Hsing Chou, Yun-Long Tsai, Hsiao-Fen Grace Chang, Pei-Yu Alison Lee. 2018. Validation of the Pockit Dengue Virus Reagent Set for Rapid Detection of Dengue Virus in Human Serum on a Field-Deployable PCR System. J Clin Microbiol 56:e01865-17.


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Wei-Kung Wang, Duane J. Gubler. 2018. Potential Point-of-Care Testing for Dengue Virus in the Field. J Clin Microbiol 56:e00203-18.


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K.L. Cooke, P. Frenzer, S.J. Tucker, P.C. Crawford, S.K. Kirk, and J.K. Levy. Rapid Diagnosis of Babesia gibsoni by Point-of-Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection. Journal of Veterinary Internal Medicine (2018) 32:232-235.


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2017

Mariano Carossino, Yanqiu Li, Pei-Yu A. Lee, Chuan-Fu Tsai, Pin-Hsing Chou, Dennis Williams, Ashley Skillman, R. Frank Cook, Grayson Brown, Hsiao-Fen G. Chang, Hwa-Tang T. Wang and Udeni B. R. Balasuriya. Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus. BMC Infectious Diseases (2017) 17:778.


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Yun Young Go, Yeon-Sook Kim, Shinhye Cheon, Sangwoo Nam, Keun Bon Ku, Meehyein Kim, Nam Hyuk Cho, Hyun Park, Pei-Yu Alison Lee, Yu-Chun Lin, Yun-Long Tsai, Hwa-Tang Thomas Wang, and Udeni B.R. Balasuriya. Evaluation and Clinical Validation of Two Field-deployable Reverse Transcription-Insulated Isothermal PCR Assays for the Detection of the Middle East Respiratory Syndrome Coronavirus. Journal of Molecular Diagnostics (2017) 19:817-827


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Rebecca P Wilkes, Eman Anis, Dawn Dunbar, Pei-Yu A Lee, Yun-Long Tsai, Fu-Chun Lee, Hsiao-Fen G Chang, Hwa-Tang T Wang and Elizabeth M Graham. Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection. Journal of Feline Medicine and Surgery 1-8 2017.


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Udeni B.R. Balasuriya, Pei-Yu Alison Lee, Yun-Long Tsai, Chuan-Fu Tsai, Yu-Han Shen, Hsiao-Fen Grace Chang, Ashley Skillman, Hwa-Tang Thomas Wang, Stéphane Pronost, Yan Zhang. 2017. Translation of a laboratory-validated equine herpesvirus-1 specific real-time PCR assay into an insulated isothermal polymerase chain reaction (iiPCR) assay for point-of-need diagnosis using POCKITTM nucleic acid analyzer. J Virol Methods. 241:58-63.


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Hung-Chih Kuo , Dan-Yuan Lo , Chiu-Lin Chen , Chien-Hsien Lee , Yu-Han Shen, Yung-Long Tsai , Pei-Yu Alison Lee * and Hsiao-Feng Grace Chang. Rapid Detection of Type 2 Porcine Reproductive and Respiratory Syndrome Virus by a Duplex Reverse Transcription Insulated Isothermal PCR on a Field-Deployable System. Jounal of Veterinary Science & Technology 2017, 8:1.


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2016

Kek Heng Chua, Ping Chin Lee and Hwa Chia Chai. Development of insulated isothermal PCR for rapid on‑site malaria detection. Malar J. 2016 Mar 1; 15:134.


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Balasuriya B.R. , Carossino M., Lee P.Y., Nam B., Skillman A., Shuck K., Timoney P., Tsai Y.L., Ma L.J., Chang H.F., and Wang H.T. Development and Evaluation of a Reverse Transcription-Insulated Isothermal Polymerase Chain Reaction (RT-iiPCR) Assay for Detection of Equine Arteritis Virus in Equine Semen and Tissue Samples using the POCKIT System. J Virol Methods. 2016 Aug;234:7-15.


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Go YY, Rajapakse J, Kularatne S, Lee PY, Ku KB, Nam S, Chou PH, Tsai YL, Liu YL, Chang HF, Wang HT and Balasuriya BR. A pan-dengue virus reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay intended for point-of-need diagnosis of dengue infection using POCKIT Nucleic Acid Analyzer. J Clin Microbiol. 2016 Jun;54(6):1528-35.


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Zhang JQ, Tsai YL, Lee PY, Chen Q, Zhang Y, Chiang CJ, Shen YH, Li FC, Chang HF, Gauger PC, Harmon KM, Wang HT. Evaluation of Two Singleplex Reverse Transcription-Insulated Isothermal PCR Tests and a Duplex Real-Time RT-PCR Test for the Detection of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus. Jounal of Virological Methods, 2016, 234, 34-42.


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Wilkes, RP et al. Comparison Between Electron Microscopy, ELISA, Real Time RT-PCR and Insulated Isothermal RT-PCR for the Detection of Rotavirus Group A (RVA) in Feces of Different Animal Species. Jounal of Virological Methods 2016 (in press)


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Kuo HC, Lo DY, Chen CL, Tsai YL, Ping JF, Lee CH, Lee PY, Chang HFC. Rapid and sensitive detection of Mycoplasma synoviae by an insulated isothermal polymerase chain reaction-based assay on a field-deployable device. Poultry Science, 2016, 0:1–7.


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Tung HY, Wang SH, Chiang YC, Tsai MS. Rapid Screening of Roundup Ready Soybean in Food Samples by a Hand-held PCR Device. Food Sci. Biotechnol. 25(4): 1-7 (2016)


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Ying-Hong Lin, Yi-Jia Lin, Li-Ling Hong, Tzu-Yu Chen, Pi-Fang Linda Chang, Development of a TaqMan Probe-Based Insulated Isothermal Polymerase Chain Reaction (iiPCR) Assay for Detection of Fusarium oxysporum f. sp. cubense Race 4., PLoS One.,DOI:10.1371/jounal.pone.0159681 July 22, 2016


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A. Ambagala, M. Fisher1, M. Goolia, C. Nfon, T. Furukawa-Stoffer, R. Ortega Polo, O. Lung. Field-Deployable Reverse Transcription-Insulated Isothermal PCR (RT-iiPCR) Assay for Rapid and Sensitive Detection of Foot-and-Mouth Disease Virus. Transboundary and Emerging Diseases. doi:10.1111/tbed.12554.


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2015

O. Lung, J. Pasick, M. Fisher, C. Buchanan, A. Erickson and A. Ambagala. Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus. Transbound Emerg Dis. 2015 Jan 27.


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Rebecca P. Wilkes, Pei-Yu A. Lee, Yun-Long Tsai, Chuan-Fu Tsai, Hsiu-Hui Chang, Hsiao-Fen G. Chang, Hwa-Tang T. Wang. An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need. J Virol Methods. 2015 Aug;220: 35-8.


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Rebecca Penrose Wilkes, Stephen A. Kania, Yun-Long Tsai, Pei-Yu Alison Lee, Hsiu-Hui Chang, Li-Juan Ma, Hsiao-Fen Grace Chang, Hwa-Tang Thomas Wang. Rapid and sensitive detection of Feline immunodeficiency virus using an insulated isothermal PCR-based assay with a point-of-need PCR detection platform. J Vet Diagn Invest. 2015 Jul;27(4):510-5.


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A. Ambagala, S. Pahari, M. Fisher, P-Y. A. Lee, J. Pasick, E. N. Ostlund, D. J. Johnson and O. Lung. A Rapid Field-Deployable Reverse Transcription-Insulated Isothermal Polymerase Chain Reaction Assay for Sensitive and Specific Detection of Bluetongue Virus. Transbound Emerg Dis. 2015 Jul 19.


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2014

Udeni B.R. Balasuriya, Pei-Yu Alison Lee, Ashish Tiwari, Ashley Skillman, Bora Nam, Thomas M. Chambers, Yun-Long Tsai, Li-Juan Ma, Pai-Chun Yang, Hsiao-Fen Grace Chang, Hwa-Tang Thomas Wang. Rapid detection of equine influenza virus H3N8 subtype by insulated isothermal RT-PCR (iiRT-PCR) assay using the POCKIT Nucleic Acid Analyzer. J Virol Methods. 2014 Oct; 207:66-72.


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Yun-Long Tsai, Han-Ching Wang, Chu-Fang Lo, Kathy Tang-Nelson, Donald Lightner, Bor-Rung Ou, Ai-Ling Hour, Chuan-Fu Tsai, Cheng-Chi Yen, Hsiao-Fen Grace Chang, Ping-Hua Teng, Pei-Yu Lee. Validation of a Commercial Insulated Isothermal PCR based POCKIT Test for Rapid and Easy Detection of White Spot Syndrome Virus Infection in Litopenaeus vannamei. PLoS One. 2014 Mar 13; 9(3):e90545.


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Rebecca P Wilkes, Yun-Long Tsai, Pei-Yu Lee, Fu-Chun Lee, Hsiao-Fen Grace Chang and Hwa-Tang Thomas Wang. Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction. BMC Vet Res. 2014 Sep 9;10:213.


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Udeni B. R. Balasuriya. Type A influenza virus detection from horsed by real-time RT-PCR and insulated RT-iiPCR. Methods Mol Biol. 2014;1161:393-402.


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2013

Hau-Yang Tsen, Chia-Ming Shih, Ping-Hua Teng, Hsin-Yen Chen, Chia-Wei Lin, Chien-Shun Chiou, Hwa-Tang Thomas Wang, Hsiao-Fen Grace Chang, Te-Yu Chung, Pei-Yu Lee, and Yu-Cheng Chiang. Detection of Salmonella in Chicken Meat by Insulated Isothermal PCR. J Food Prot. 2013 Aug;76(8):1322-9.


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2012

Yun-Long Tsai, Yu-Chan Lin, Pin-Hsing Chou, Ping-Hua Teng, Pei-Yu Lee. Detection of white spot syndrome virus by polymerase chain reaction performed under insulated isothermal conditions. J Virol Methods 2012 Apr 4; 181(1):134-7.


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Yun-Long Tsai, Hwa-Tang Thomas Wang, Hsiao-Fen Grace Chang, Chuan-Fu Tsai, Ching-Ko Lin, Ping-Hua Teng, Chen Su, Chien-Chung Jeng, Pei-Yu Lee. Development of TaqMan Probe-Based Insulated Isothermal PCR (iiPCR) for Sensitive and Specific On-Site Pathogen Detection. PLoS One 2012 25; 7(9):e45278.


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Hsiao-Fen Grace Chang, Yun-Long Tsai, Chuan-Fu Tsai, Ching-Ko Lin, Pei-Yu Lee, Ping-Hua Teng, Chen Su and Chien-Chung Jeng .A thermally baffled device for highly stabilized convective PCR. Biotechnol J. 2012 May;7(5):662-6.


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