Biodetection Technologies for First Responders

2015

Biodetection Technologies for First Responders: 2015 edition. PCR-based Detection Systems: GeneReach USA: POCKIT™. p 5.11-12. Pacific Northwest National Laboratory.(PNNL)

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2014

Biodetection Technologies for First Responders: 2014 edition. PCR-based Detection Systems: GeneReach USA: POCKIT™. p 5.10. Pacific Northwest National Laboratory.(PNNL)

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SCI Publications

2018

Jih-Jin Tsai, Li-Teh Liu, Ping-Chang Lin, Ching-Yi Tsai, Pin-Hsing Chou, Yun-Long Tsai, Hsiao-Fen Grace Chang, Pei-Yu Alison Lee. 2018. Validation of the Pockit Dengue Virus Reagent Set for Rapid Detection of Dengue Virus in Human Serum on a Field-Deployable PCR System. J Clin Microbiol 56:e01865-17.

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Vissani M.A., Tordoya M.S., Tsai Y.-L., Lee P.-Y.A., Shen Y.-H., Lee F.-C., Wang H.-T.T., Parreño V., Barrandeguy M. 2018. On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions. Journal of Virological Methods 257 (2018) 29–32

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Wei-Kung Wang, Duane J. Gubler. 2018. Potential Point-of-Care Testing for Dengue Virus in the Field. J Clin Microbiol 56:e00203-18.

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K.L. Cooke, P. Frenzer, S.J. Tucker, P.C. Crawford, S.K. Kirk, and J.K. Levy. Rapid Diagnosis of Babesia gibsoni by Point-of-Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection. Journal of Veterinary Internal Medicine (2018) 32:232-235

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2017

Go YY, Kim YS, Cheon S, Ku KB, Nam SW, Kim M, Lee P-YA, Lin Y-C, Tsai Y-L, 1146 Wang H-TT et al: Evaluation and clinical validation of a field-deployable Reverse Transcription-Insulated Isothermal PCR Assay for the Detection of the Middle East Respiratory Syndrome Coronavirus. J Mol Diagn 2017;19(6):817–827.

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Mariano Carossino, Yanqiu Li, Pei-Yu A. Lee, Chuan-Fu Tsai, Pin-Hsing Chou, Dennis Williams, Ashley Skillman, R. Frank Cook, Grayson Brown, Hsiao-Fen G. Chang, Hwa-Tang T. Wang and Udeni B. R. Balasuriya. Evaluation of a field-deployable reverse transcription-insulated isothermal PCR for rapid and sensitive on-site detection of Zika virus. BMC Infectious Diseases (2017) 17:778.

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Rebecca P Wilkes, Eman Anis, Dawn Dunbar, Pei-Yu A Lee, Yun-Long Tsai, Fu-Chun Lee, Hsiao-Fen G Chang, Hwa-Tang T Wang and Elizabeth M Graham. Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection. Journal of Feline Medicine and Surgery 1-8 2017.

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Udeni B.R. Balasuriya, Pei-Yu Alison Lee, Yun-Long Tsai, Chuan-Fu Tsai, Yu-Han Shen, Hsiao-Fen Grace Chang, Ashley Skillman, Hwa-Tang Thomas Wang, Stéphane Pronost, Yan Zhang. 2017. Translation of a laboratory-validated equine herpesvirus-1 specific real-time PCR assay into an insulated isothermal polymerase chain reaction (iiPCR) assay for point-of-need diagnosis using POCKIT^TM nucleic acid analyzer. J Virol Methods. 241:58-63.

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Hung-Chih Kuo , Dan-Yuan Lo , Chiu-Lin Chen , Chien-Hsien Lee , Yu-Han Shen, Yung-Long Tsai , Pei-Yu Alison Lee * and Hsiao-Feng Grace Chang. Rapid Detection of Type 2 Porcine Reproductive and Respiratory Syndrome Virus by a Duplex Reverse Transcription Insulated Isothermal PCR on a Field-Deployable System. Jounal of Veterinary Science & Technology 2017, 8:1.

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2016

Kek Heng Chua, Ping Chin Lee and Hwa Chia Chai. Development of insulated isothermal PCR for rapid on‑site malaria detection. Malar J. 2016 Mar 1; 15:134.

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Balasuriya B.R. , Carossino M., Lee P.Y., Nam B., Skillman A., Shuck K., Timoney P., Tsai Y.L., Ma L.J., Chang H.F., and Wang H.T. Development and Evaluation of a Reverse Transcription-Insulated Isothermal Polymerase Chain Reaction (RT-iiPCR) Assay for Detection of Equine Arteritis Virus in Equine Semen and Tissue Samples using the POCKIT System. J Virol Methods. 2016 Aug;234:7-15.

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Go YY, Rajapakse J, Kularatne S, Lee PY, Ku KB, Nam S, Chou PH, Tsai YL, Liu YL, Chang HF, Wang HT and Balasuriya BR. A pan-dengue virus reverse transcription-insulated isothermal polymerase chain reaction (RT-iiPCR) assay intended for point-of-need diagnosis of dengue infection using POCKIT Nucleic Acid Analyzer. J Clin Microbiol. 2016 Jun;54(6):1528-35.

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Zhang JQ, Tsai YL, Lee PY, Chen Q, Zhang Y, Chiang CJ, Shen YH, Li FC, Chang HF, Gauger PC, Harmon KM, Wang HT. Evaluation of Two Singleplex Reverse Transcription-Insulated Isothermal PCR Tests and a Duplex Real-Time RT-PCR Test for the Detection of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus. Jounal of Virological Methods, 2016, 234, 34-42.

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Wilkes, RP et al. Comparison Between Electron Microscopy, ELISA, Real Time RT-PCR and Insulated Isothermal RT-PCR for the Detection of Rotavirus Group A (RVA) in Feces of Different Animal Species. Jounal of Virological Methods 2016 (in press)

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Kuo HC, Lo DY, Chen CL, Tsai YL, Ping JF, Lee CH, Lee PY, Chang HFC. Rapid and sensitive detection of Mycoplasma synoviae by an insulated isothermal polymerase chain reaction-based assay on a ﬁeld-deployable device. Poultry Science, 2016, 0:1–7.

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Tung HY, Wang SH, Chiang YC, Tsai MS. Rapid Screening of Roundup Ready Soybean in Food Samples by a Hand-held PCR Device. Food Sci. Biotechnol. 25(4): 1-7 (2016)

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Ying-Hong Lin, Yi-Jia Lin, Li-Ling Hong, Tzu-Yu Chen, Pi-Fang Linda Chang, Development of a TaqMan Probe-Based Insulated Isothermal Polymerase Chain Reaction (iiPCR) Assay for Detection of Fusarium oxysporum f. sp. cubense Race 4., PLoS One.,DOI:10.1371/jounal.pone.0159681 July 22, 2016

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A. Ambagala, M. Fisher1, M. Goolia, C. Nfon, T. Furukawa-Stoffer, R. Ortega Polo, O. Lung. Field-Deployable Reverse Transcription-Insulated Isothermal PCR (RT-iiPCR) Assay for Rapid and Sensitive Detection of Foot-and-Mouth Disease Virus. Transboundary and Emerging Diseases. doi:10.1111/tbed.12554.

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2015

O. Lung, J. Pasick, M. Fisher, C. Buchanan, A. Erickson and A. Ambagala. Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus. Transbound Emerg Dis. 2015 Jan 27.

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Rebecca P. Wilkes, Pei-Yu A. Lee, Yun-Long Tsai, Chuan-Fu Tsai, Hsiu-Hui Chang, Hsiao-Fen G. Chang, Hwa-Tang T. Wang. An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need. J Virol Methods. 2015 Aug;220: 35-8.

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Rebecca Penrose Wilkes, Stephen A. Kania, Yun-Long Tsai, Pei-Yu Alison Lee, Hsiu-Hui Chang, Li-Juan Ma, Hsiao-Fen Grace Chang, Hwa-Tang Thomas Wang. Rapid and sensitive detection of Feline immunodeficiency virus using an insulated isothermal PCR-based assay with a point-of-need PCR detection platform. J Vet Diagn Invest. 2015 Jul;27(4):510-5.

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A. Ambagala, S. Pahari, M. Fisher, P-Y. A. Lee, J. Pasick, E. N. Ostlund, D. J. Johnson and O. Lung. A Rapid Field-Deployable Reverse Transcription-Insulated Isothermal Polymerase Chain Reaction Assay for Sensitive and Specific Detection of Bluetongue Virus. Transbound Emerg Dis. 2015 Jul 19.

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2014

Udeni B.R. Balasuriya, Pei-Yu Alison Lee, Ashish Tiwari, Ashley Skillman, Bora Nam, Thomas M. Chambers, Yun-Long Tsai, Li-Juan Ma, Pai-Chun Yang, Hsiao-Fen Grace Chang, Hwa-Tang Thomas Wang. Rapid detection of equine influenza virus H3N8 subtype by insulated isothermal RT-PCR (iiRT-PCR) assay using the POCKIT Nucleic Acid Analyzer. J Virol Methods. 2014 Oct; 207:66-72.

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Yun-Long Tsai, Han-Ching Wang, Chu-Fang Lo, Kathy Tang-Nelson, Donald Lightner, Bor-Rung Ou, Ai-Ling Hour, Chuan-Fu Tsai, Cheng-Chi Yen, Hsiao-Fen Grace Chang, Ping-Hua Teng, Pei-Yu Lee. Validation of a Commercial Insulated Isothermal PCR based POCKIT Test for Rapid and Easy Detection of White Spot Syndrome Virus Infection in Litopenaeus vannamei. PLoS One. 2014 Mar 13; 9(3):e90545.

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Rebecca P Wilkes, Yun-Long Tsai, Pei-Yu Lee, Fu-Chun Lee, Hsiao-Fen Grace Chang and Hwa-Tang Thomas Wang. Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction. BMC Vet Res. 2014 Sep 9;10:213.

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Udeni B. R. Balasuriya. Type A influenza virus detection from horsed by real-time RT-PCR and insulated RT-iiPCR. Methods Mol Biol. 2014;1161:393-402.

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2013

Hau-Yang Tsen, Chia-Ming Shih, Ping-Hua Teng, Hsin-Yen Chen, Chia-Wei Lin, Chien-Shun Chiou, Hwa-Tang Thomas Wang, Hsiao-Fen Grace Chang, Te-Yu Chung, Pei-Yu Lee, and Yu-Cheng Chiang. Detection of Salmonella in Chicken Meat by Insulated Isothermal PCR. J Food Prot. 2013 Aug;76(8):1322-9

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2012

Yun-Long Tsai, Yu-Chan Lin, Pin-Hsing Chou, Ping-Hua Teng, Pei-Yu Lee. Detection of white spot syndrome virus by polymerase chain reaction performed under insulated isothermal conditions. J Virol Methods 2012 Apr 4; 181(1):134-7.

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Yun-Long Tsai, Hwa-Tang Thomas Wang, Hsiao-Fen Grace Chang, Chuan-Fu Tsai, Ching-Ko Lin, Ping-Hua Teng, Chen Su, Chien-Chung Jeng, Pei-Yu Lee. Development of TaqMan Probe-Based Insulated Isothermal PCR (iiPCR) for Sensitive and Specific On-Site Pathogen Detection. PLoS One 2012 25; 7(9):e45278.

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Hsiao-Fen Grace Chang, Yun-Long Tsai, Chuan-Fu Tsai, Ching-Ko Lin, Pei-Yu Lee, Ping-Hua Teng, Chen Su and Chien-Chung Jeng .A thermally baffled device for highly stabilized convective PCR. Biotechnol J. 2012 May;7(5):662-6.

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PRODUCTS

Biodetection Technologies for First Responders

Biodetection Technologies for First Responders: 2015 edition. PCR-based Detection Systems: GeneReach USA: POCKIT™. p 5.11-12. Pacific Northwest National Laboratory.(PNNL)

Biodetection Technologies for First Responders: 2014 edition. PCR-based Detection Systems: GeneReach USA: POCKIT™. p 5.10. Pacific Northwest National Laboratory.(PNNL)

SCI Publications

Vissani M.A., Tordoya M.S., Tsai Y.-L., Lee P.-Y.A., Shen Y.-H., Lee F.-C., Wang H.-T.T., Parreño V., Barrandeguy M. 2018. On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions. Journal of Virological Methods 257 (2018) 29–32

Wei-Kung Wang, Duane J. Gubler. 2018. Potential Point-of-Care Testing for Dengue Virus in the Field. J Clin Microbiol 56:e00203-18.

K.L. Cooke, P. Frenzer, S.J. Tucker, P.C. Crawford, S.K. Kirk, and J.K. Levy. Rapid Diagnosis of Babesia gibsoni by Point-of-Need Testing by Insulated Isothermal PCR in Dogs at High Risk of Infection. Journal of Veterinary Internal Medicine (2018) 32:232-235

Rebecca P Wilkes, Eman Anis, Dawn Dunbar, Pei-Yu A Lee, Yun-Long Tsai, Fu-Chun Lee, Hsiao-Fen G Chang, Hwa-Tang T Wang and Elizabeth M Graham. Rapid and sensitive insulated isothermal PCR for point-of-need feline leukaemia virus detection. Journal of Feline Medicine and Surgery 1-8 2017.

Kek Heng Chua, Ping Chin Lee and Hwa Chia Chai. Development of insulated isothermal PCR for rapid on‑site malaria detection. Malar J. 2016 Mar 1; 15:134.

Wilkes, RP et al. Comparison Between Electron Microscopy, ELISA, Real Time RT-PCR and Insulated Isothermal RT-PCR for the Detection of Rotavirus Group A (RVA) in Feces of Different Animal Species. Jounal of Virological Methods 2016 (in press)

Kuo HC, Lo DY, Chen CL, Tsai YL, Ping JF, Lee CH, Lee PY, Chang HFC. Rapid and sensitive detection of Mycoplasma synoviae by an insulated isothermal polymerase chain reaction-based assay on a ﬁeld-deployable device. Poultry Science, 2016, 0:1–7.

Tung HY, Wang SH, Chiang YC, Tsai MS. Rapid Screening of Roundup Ready Soybean in Food Samples by a Hand-held PCR Device. Food Sci. Biotechnol. 25(4): 1-7 (2016)

Ying-Hong Lin, Yi-Jia Lin, Li-Ling Hong, Tzu-Yu Chen, Pi-Fang Linda Chang, Development of a TaqMan Probe-Based Insulated Isothermal Polymerase Chain Reaction (iiPCR) Assay for Detection of Fusarium oxysporum f. sp. cubense Race 4., PLoS One.,DOI:10.1371/jounal.pone.0159681 July 22, 2016

A. Ambagala, M. Fisher1, M. Goolia, C. Nfon, T. Furukawa-Stoffer, R. Ortega Polo, O. Lung. Field-Deployable Reverse Transcription-Insulated Isothermal PCR (RT-iiPCR) Assay for Rapid and Sensitive Detection of Foot-and-Mouth Disease Virus. Transboundary and Emerging Diseases. doi:10.1111/tbed.12554.

O. Lung, J. Pasick, M. Fisher, C. Buchanan, A. Erickson and A. Ambagala. Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus. Transbound Emerg Dis. 2015 Jan 27.

Rebecca P. Wilkes, Pei-Yu A. Lee, Yun-Long Tsai, Chuan-Fu Tsai, Hsiu-Hui Chang, Hsiao-Fen G. Chang, Hwa-Tang T. Wang. An insulated isothermal PCR method on a field-deployable device for rapid and sensitive detection of canine parvovirus type 2 at points of need. J Virol Methods. 2015 Aug;220: 35-8.

A. Ambagala, S. Pahari, M. Fisher, P-Y. A. Lee, J. Pasick, E. N. Ostlund, D. J. Johnson and O. Lung. A Rapid Field-Deployable Reverse Transcription-Insulated Isothermal Polymerase Chain Reaction Assay for Sensitive and Specific Detection of Bluetongue Virus. Transbound Emerg Dis. 2015 Jul 19.

Rebecca P Wilkes, Yun-Long Tsai, Pei-Yu Lee, Fu-Chun Lee, Hsiao-Fen Grace Chang and Hwa-Tang Thomas Wang. Rapid and sensitive detection of canine distemper virus by one-tube reverse transcription-insulated isothermal polymerase chain reaction. BMC Vet Res. 2014 Sep 9;10:213.

Udeni B. R. Balasuriya. Type A influenza virus detection from horsed by real-time RT-PCR and insulated RT-iiPCR. Methods Mol Biol. 2014;1161:393-402.

Hau-Yang Tsen, Chia-Ming Shih, Ping-Hua Teng, Hsin-Yen Chen, Chia-Wei Lin, Chien-Shun Chiou, Hwa-Tang Thomas Wang, Hsiao-Fen Grace Chang, Te-Yu Chung, Pei-Yu Lee, and Yu-Cheng Chiang. Detection of Salmonella in Chicken Meat by Insulated Isothermal PCR. J Food Prot. 2013 Aug;76(8):1322-9

Yun-Long Tsai, Yu-Chan Lin, Pin-Hsing Chou, Ping-Hua Teng, Pei-Yu Lee. Detection of white spot syndrome virus by polymerase chain reaction performed under insulated isothermal conditions. J Virol Methods 2012 Apr 4; 181(1):134-7.

Yun-Long Tsai, Hwa-Tang Thomas Wang, Hsiao-Fen Grace Chang, Chuan-Fu Tsai, Ching-Ko Lin, Ping-Hua Teng, Chen Su, Chien-Chung Jeng, Pei-Yu Lee. Development of TaqMan Probe-Based Insulated Isothermal PCR (iiPCR) for Sensitive and Specific On-Site Pathogen Detection. PLoS One 2012 25; 7(9):e45278.

Hsiao-Fen Grace Chang, Yun-Long Tsai, Chuan-Fu Tsai, Ching-Ko Lin, Pei-Yu Lee, Ping-Hua Teng, Chen Su and Chien-Chung Jeng .A thermally baffled device for highly stabilized convective PCR. Biotechnol J. 2012 May;7(5):662-6.