Real-time PCR system for detection of Vibrio cholerae
Introduction
PCR technology has been commonly employed in the aquaculture industry for disease diagnosis and prevention. But with the growing demands for a quantitative research tool and requirement for a high throughput screening system, real-time PCR is receiving more attention and becoming more important in the industry than before. GeneReach has developed IQ REAL™ Vibrio cholerae Quantitative System a real-time PCR-based diagnostic system. Its advanced designs, listed below, provide an accurate, sensitive, and flexible real-time PCR system for Vibrio cholera (V. cholera) detection in the shrimp culture industry.
Advantages of IQ REAL™ Vibrio cholerae Quantitative System：
- TaqMan® assay strategy: This system can tolerate higher sample loads than SYBR Green based reaction. Consequently, it is able to detect samples of very low target/shrimp cell ratio.
- Dual color design: This system can quantify both target pathogen (FAM) and shrimp (VIC) genomic nucleic acid in a single reaction. The built-in internal control (VIC) prevents any false negative interpretation of results from failed extraction or inhibition.
